RANTES Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1324R-BF488-100uL |
| Product Name | RANTES Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Pig |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CCL5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6352 |
| Swiss Prot | P13501 |
| Subcellular location | Secreted |
| Synonyms | SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta; C-C motif chemokine 5; Eosinophil chemotactic cytokine; Small-inducible cytokine A5; T cell-specific protein P228; T-cell-specific protein RANTES; CCL5 |
| Background | Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils (PubMed:1679162, PubMed:13864, PubMed:8525373, PubMed:9516414, PubMed:15923218). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RANTES Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1324R-BF488) validated for, and at what dilution?
bs-1324R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RANTES Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1324R-BF488) react with?
Tested reactivity: Human, Mouse, Pig. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Rabbit.
How should bs-1324R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RANTES Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1324R-BF488)?
KLH conjugated synthetic peptide derived from human CCL5
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-1324R-BF488)?
AbBy Fluor-488 on bs-1324R-BF488: excitation / emission 499nm/519nm.