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Tenascin C Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-1327R-PerCP-100uL
Product Name Tenascin C Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Chicken, Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Tenascin C
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3371
Swiss Prot P24821
Subcellular location Extracellular matrix
Synonyms Cytotactin; Glioma associated extracellular matrix antigen; GMEM; GP 150 225; Hexabrachion; HXB; JI; Myotendinous antigen; Neuronectin; Tenascin C; TenascinC; TN; TN C; TNC.Tn-C; Tenascin-C.
Background Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Tenascin C Polyclonal Antibody, PerCP Conjugated (bs-1327R-PerCP) validated for, and at what dilution?

bs-1327R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Tenascin C Polyclonal Antibody, PerCP Conjugated (bs-1327R-PerCP) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Chicken, Rabbit.

How should bs-1327R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Tenascin C Polyclonal Antibody, PerCP Conjugated (bs-1327R-PerCP)?

KLH conjugated synthetic peptide derived from human Tenascin C

What are the excitation and emission wavelengths of the PerCP conjugate (bs-1327R-PerCP)?

PerCP on bs-1327R-PerCP: excitation / emission 482nm/677nm.

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