ZBED5 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-13555R-Cy3-100uL |
| Product Name | ZBED5 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Horse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZBED5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 58486 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Transposon derived Buster 1 transposase like protein; Zinc finger BED type containing 5; ZBED5_HUMAN. |
| Background | ZBED5 contains 1 BED type zinc finger and it may be derived from an ancient transposon that has lost its ability to translocate. This gene is unusual in that its coding sequence is mostly derived from Charlie like DNA transposon. There is mRNA and EST evidence to suggest that this gene is transcribed. The encoded protein shares 70% identity with Charlie 1 transposase, however, this gene does not appear to be an active DNA transposon as it is not flanked by terminal inverted repeats. The exact function of this gene product is not known. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZBED5 Polyclonal Antibody, Cy3 Conjugated (bs-13555R-Cy3) validated for, and at what dilution?
bs-13555R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-13555R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZBED5 Polyclonal Antibody, Cy3 Conjugated (bs-13555R-Cy3)?
KLH conjugated synthetic peptide derived from human ZBED5
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-13555R-Cy3)?
Cy3 on bs-13555R-Cy3: excitation / emission 512,550nm/570,615nm.