ZBTB5 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-13581R-FITC-100uL |
| Product Name | ZBTB5 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZBTB5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9925 |
| Subcellular location | Nucleus |
| Synonyms | 5930421I10; 9430083K24Rik; AI646847; Expressed in brain and neural tube; KIAA0354; mKIAA0354; OTTMUSP00000007812; OTTMUSP00000007813; OTTMUSP00000007814; RP11-397D12.5; RP23-218A4.2; Transcription factor ZNF POZ; Zbtb5; ZBTB5_HUMAN; Zinc finger and BTB domain containing protein 5; Zinc finger and BTB domain-containing protein 5. |
| Background | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kr_ppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Zinc finger and BTB domain-containing protein 5 (ZBTB5) is a 677 amino acid member of the Kr_ppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZBTB5 contains a BTB domain, also known as a POZ domain, which inhibits DNA binding and mediates homotypic and heterotypic dimerization. Characteristics of the BTB domain suggest that ZBTB5 functions as a transcription regulator. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZBTB5 Polyclonal Antibody, FITC Conjugated (bs-13581R-FITC) validated for, and at what dilution?
bs-13581R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZBTB5 Polyclonal Antibody, FITC Conjugated (bs-13581R-FITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit.
How should bs-13581R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZBTB5 Polyclonal Antibody, FITC Conjugated (bs-13581R-FITC)?
KLH conjugated synthetic peptide derived from human ZBTB5
What are the excitation and emission wavelengths of the FITC conjugate (bs-13581R-FITC)?
FITC on bs-13581R-FITC: excitation / emission 494nm/518nm.