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ZBTB5 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-13581R-PE-100uL
Product Name ZBTB5 Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ZBTB5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9925
Subcellular location Nucleus
Synonyms 5930421I10; 9430083K24Rik; AI646847; Expressed in brain and neural tube; KIAA0354; mKIAA0354; OTTMUSP00000007812; OTTMUSP00000007813; OTTMUSP00000007814; RP11-397D12.5; RP23-218A4.2; Transcription factor ZNF POZ; Zbtb5; ZBTB5_HUMAN; Zinc finger and BTB domain containing protein 5; Zinc finger and BTB domain-containing protein 5.
Background Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kr_ppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Zinc finger and BTB domain-containing protein 5 (ZBTB5) is a 677 amino acid member of the Kr_ppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZBTB5 contains a BTB domain, also known as a POZ domain, which inhibits DNA binding and mediates homotypic and heterotypic dimerization. Characteristics of the BTB domain suggest that ZBTB5 functions as a transcription regulator.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is ZBTB5 Polyclonal Antibody, PE Conjugated (bs-13581R-PE) validated for, and at what dilution?

bs-13581R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ZBTB5 Polyclonal Antibody, PE Conjugated (bs-13581R-PE) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit.

How should bs-13581R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ZBTB5 Polyclonal Antibody, PE Conjugated (bs-13581R-PE)?

KLH conjugated synthetic peptide derived from human ZBTB5

What are the excitation and emission wavelengths of the PE conjugate (bs-13581R-PE)?

PE on bs-13581R-PE: excitation / emission 496,564nm/578nm.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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