BMP4 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1374R-PerCP-Cy5.5-100uL |
| Product Name | BMP4 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Mouse, Sheep |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat BMP4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | BMP 4; BMP2B; BMP2B1; Bone morphogenetic protein 4; DVR4; ZYME; BMP4_HUMAN; BMP-4; Bone morphogenetic protein 2B; BMP-2B. |
| Background | BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is BMP4 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-1374R-PERCP-CY5-5) validated for, and at what dilution?
BS-1374R-PERCP-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BMP4 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-1374R-PERCP-CY5-5) react with?
Tested reactivity: Mouse, Sheep. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should BS-1374R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BMP4 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-1374R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from rat BMP4
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-1374R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-1374R-PERCP-CY5-5: excitation / emission 482nm/694nm.