H2BFWT Polyclonal Antibody, RBITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-15401R-RBITC-100uL |
| Product Name | H2BFWT Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human H2BFWT |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 158983 |
| Subcellular location | Nucleus |
| Synonyms | H2B histone family member W testis-specic; H2BFWT; H2BWT_HUMAN; Histone H2B type W-T. |
| Background | H2BFWT (H2B histone family member W testis-specific) is a 175 amino acid nuclear membrane histone that belongs to the histone H2B family. In contrast to most H2B histones, H2BFWT does not contain the conserved C-terminal residue involved in monoubiquitination, but is structurally indistinguishable from conventional H2B histones. Also varying from other H2B histones, H2BFWT does not participate in the recruitment of chromosome condensation factors or in the assembly of mitotic chromsomes. Expressed in testis, H2BFWT is present in sperm cells and may be essential to telomere function. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is H2BFWT Polyclonal Antibody, RBITC Conjugated (bs-15401R-RBITC) validated for, and at what dilution?
bs-15401R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-15401R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise H2BFWT Polyclonal Antibody, RBITC Conjugated (bs-15401R-RBITC)?
KLH conjugated synthetic peptide derived from human H2BFWT
What are the excitation and emission wavelengths of the RBITC conjugate (bs-15401R-RBITC)?
RBITC on bs-15401R-RBITC: excitation / emission 540nm/625nm.