XBP1 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1668R-Cy5-100uL |
| Product Name | XBP1 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | XBP-1 antibody will recognize both isoforms, may also interact with chicken |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human XBP-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7494 |
| Swiss Prot | P17861 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | XBP2; TREB5; XBP-1; X-box-binding protein 1; Tax-responsive element-binding protein 5; XBP1 |
| Background | Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is XBP1 Polyclonal Antibody, Cy5 Conjugated (bs-1668R-Cy5) validated for, and at what dilution?
bs-1668R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does XBP1 Polyclonal Antibody, Cy5 Conjugated (bs-1668R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Chicken.
How should bs-1668R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise XBP1 Polyclonal Antibody, Cy5 Conjugated (bs-1668R-Cy5)?
KLH conjugated synthetic peptide derived from human XBP-1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-1668R-Cy5)?
Cy5 on bs-1668R-Cy5: excitation / emission 625,650nm/670nm.