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RPS20 Polyclonal Antibody, PerCP-Cy5.5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat
  • Yeast

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-18322R-PerCP-Cy5.5-100uL
Product Name RPS20 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat, Yeast
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation PerCP-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RPS20
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6224
Swiss Prot P60866
Subcellular location Cytoplasm
Synonyms S20; 40S ribosomal protein S20; RPS20
Background Mammalian ribosomal proteins are encoded by multigene families that consist of processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S20, also known as RPS20, is a 119 amino acid cytoplasmic protein that is a component of the 40S ribosomal subunit. Co-transcribed with the small nucleolar RNA gene U54, Ribosomal Protein S20 is a primary binding protein (it binds independently to its target protein) that interacts with both the 5 and 3 minor domains of 16S ribosomal RNA (rRNA). Through its interactions with 16S rRNA, Ribosomal Protein S20 is thought to play a key role in nucleating the assembly of the 30S ribosomal subunit. Like most ribosomal protein-coding genes, the gene encoding Ribosomal Protein S20 is dispersed throughout the genome and exists as multiple processed pseudogenes.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is RPS20 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-18322R-PERCP-CY5-5) validated for, and at what dilution?

BS-18322R-PERCP-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RPS20 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-18322R-PERCP-CY5-5) react with?

Tested reactivity: Rat, Yeast. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse.

How should BS-18322R-PERCP-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RPS20 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-18322R-PERCP-CY5-5)?

KLH conjugated synthetic peptide derived from human RPS20

What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-18322R-PERCP-CY5-5)?

PerCP-Cy5.5 on BS-18322R-PERCP-CY5-5: excitation / emission 482nm/694nm.

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