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Serotonin transporter Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
  • GuineaPig
Overview
Catalog # bs-1893R-Cy5.5-100uL
Product Name Serotonin transporter Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Chicken, GuineaPig
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human 5-HTT
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6532
Swiss Prot P31645
Subcellular location Cell membrane
Synonyms HTT; 5HTT; OCD1; SERT; 5-HTT; SERT1; hSERT; 5-HTTLPR; Sodium-dependent serotonin transporter; 5HT transporter; Solute carrier family 6 member 4; SLC6A4
Background Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is Serotonin transporter Polyclonal Antibody, Cy5.5 Conjugated (BS-1893R-CY5-5) validated for, and at what dilution?

BS-1893R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Serotonin transporter Polyclonal Antibody, Cy5.5 Conjugated (BS-1893R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Chicken, GuineaPig.

How should BS-1893R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Serotonin transporter Polyclonal Antibody, Cy5.5 Conjugated (BS-1893R-CY5-5)?

KLH conjugated synthetic peptide derived from human 5-HTT

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-1893R-CY5-5)?

Cy5.5 on BS-1893R-CY5-5: excitation / emission 675nm/694nm.

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