ZAP70 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1942R-Biotin-100uL |
| Product Name | ZAP70 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-F
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZAP70 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 7535 |
| Swiss Prot | P43403 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ZAP-70; ZAP 70; ZAP70; zeta-associated protein 70; ZAP-70=protein tyrosine kinase Syk homolog {SH2-like and C-terminal kinase domains}; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-associated protein; Syk-related tyrosine kinase. SRK; STD; TZK; ZAP70_HUMAN; Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein. |
| Background | This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is ZAP70 Polyclonal Antibody, Biotin Conjugated (bs-1942R-Biotin) validated for, and at what dilution?
bs-1942R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZAP70 Polyclonal Antibody, Biotin Conjugated (bs-1942R-Biotin) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should bs-1942R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZAP70 Polyclonal Antibody, Biotin Conjugated (bs-1942R-Biotin)?
KLH conjugated synthetic peptide derived from human ZAP70