Anillin Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20482R-HRP-100uL |
| Product Name | Anillin Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Anillin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54443 |
| Swiss Prot | Q9NQW6 |
| Synonyms | Actin binding protein scraps homolog Drosophila; Actin binding protein anillin; Anillin actin binding protein; ANLN; DKFZp779A055; Scra; Scraps; Scraps homolog.,, |
| Background | Anillin, also known as scraps homolog, is an evolutionarily conserved Actin- binding protein required for cytokinesis that was first identified in Drosophila melanogaster. Anillin is a ubiquitously expressed protein with highest expression levels in the central nervous system. It is predominantly found in the nucleus and it localizes to the cleavage furrow during cytokinesis, forming a ring with the help of Rac GTPase. During cytokinesis, Anillin interacts with CD2AP and functions to concentrate Rho A and maintain the localization of active Myosin. In Anillin knockout cells the cleavage furrow fails to complete ingression. Anillin expression levels fluctuate with the cell cycle, peaking in mitosis. Before the cell exits into G1, Anillin associates with E-cadherin and is ubiquitinated by the anaphase-promoting complex/cyclosome (APC/C). APC/C recognizes the D-box domain at the N-terminal region of Anillin. Anillin is commonly overexpressed in tumors and may serve as a potential biomarker.Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | 1:100-500 |
| IHC-F | |
Frequently asked questions
Which applications is Anillin Polyclonal Antibody, HRP Conjugated (bs-20482R-HRP) validated for, and at what dilution?
bs-20482R-HRP is validated for WB (1:500-2000); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Anillin Polyclonal Antibody, HRP Conjugated (bs-20482R-HRP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-20482R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Anillin Polyclonal Antibody, HRP Conjugated (bs-20482R-HRP)?
KLH conjugated synthetic peptide derived from human Anillin