ALKBH5 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20540R-FITC-100uL |
| Product Name | ALKBH5 Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ALKBH5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54890 |
| Swiss Prot | Q6P6C2 |
| Subcellular location | Nucleus |
| Synonyms | ABH5; AlkB, alkylation repair homolog 5 (E. coli); AlkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha ketoglutarate dependent dioxygenase alkB homolog 5; OFOXD; OFOXD1; Oxoglutarate and iron-dependent oxygenase domain containing; Probable alpha ketoglutarate dependent dioxygenase ABH5; RNA demethylase ALKBH5. |
| Background | Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A)RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178,PubMed:24616105, PubMed:24489119). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro)(PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178,PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ALKBH5 Polyclonal Antibody, FITC Conjugated (bs-20540R-FITC) validated for, and at what dilution?
bs-20540R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ALKBH5 Polyclonal Antibody, FITC Conjugated (bs-20540R-FITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Rabbit.
How should bs-20540R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ALKBH5 Polyclonal Antibody, FITC Conjugated (bs-20540R-FITC)?
KLH conjugated synthetic peptide derived from human ALKBH5
What are the excitation and emission wavelengths of the FITC conjugate (bs-20540R-FITC)?
FITC on bs-20540R-FITC: excitation / emission 494nm/518nm.