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EGFP Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Species independent
Overview
Catalog # bs-2194R-BF555-100uL
Product Name EGFP Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
WB IF(IHC-P)
Specificity This antibody will work best with EGFP that has a similar amino acid make-up as that of Aequorea Victoria.
Reactivity Species independent
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source Purified EGFP synthetic protein homologous to EGFP naturally occurring in Aequorea victoria
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms Enhanced Green Fluorescent Protein.
Background The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is EGFP Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2194R-BF555) validated for, and at what dilution?

bs-2194R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EGFP Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2194R-BF555) react with?

Tested reactivity: Species independent.

How should bs-2194R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EGFP Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2194R-BF555)?

Purified EGFP synthetic protein homologous to EGFP naturally occurring in Aequorea victoria

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-2194R-BF555)?

AbBy Fluor-555 on bs-2194R-BF555: excitation / emission 553nm/568nm.

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