EGFP Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2194R-Cy3-100uL |
| Product Name | EGFP Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Specificity | This antibody will work best with EGFP that has a similar amino acid make-up as that of Aequorea Victoria. |
| Reactivity | Species independent |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Purified EGFP synthetic protein homologous to EGFP naturally occurring in Aequorea victoria |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Enhanced Green Fluorescent Protein. |
| Background | The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is EGFP Polyclonal Antibody, Cy3 Conjugated (bs-2194R-Cy3) validated for, and at what dilution?
bs-2194R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EGFP Polyclonal Antibody, Cy3 Conjugated (bs-2194R-Cy3) react with?
Tested reactivity: Species independent.
How should bs-2194R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EGFP Polyclonal Antibody, Cy3 Conjugated (bs-2194R-Cy3)?
Purified EGFP synthetic protein homologous to EGFP naturally occurring in Aequorea victoria
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-2194R-Cy3)?
Cy3 on bs-2194R-Cy3: excitation / emission 512,550nm/570,615nm.