Zic2 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-22517R-HRP-100uL |
| Product Name | Zic2 Polyclonal Antibody, HRP Conjugated |
| Applications |
IHC-P
IHC-F
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Pig, Horse |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Zic2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7546 |
| Swiss Prot | O95409 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HPE 5; HPE5; Odd paired homolog Drosophila; Zic 2; Zic family member 2 (odd paired Drosophila homolog); Zic family member 2; ZIC2; ZIC2_HUMAN; Zinc finger protein of the cerebellum 2; Zinc finger protein ZIC 2; Zinc finger protein Zic2. |
| Background | Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos. |
| Application Dilution | |
| IHC-P | 1:200-400 |
| IHC-F | |
Frequently asked questions
Which applications is Zic2 Polyclonal Antibody, HRP Conjugated (bs-22517R-HRP) validated for, and at what dilution?
bs-22517R-HRP is validated for IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Zic2 Polyclonal Antibody, HRP Conjugated (bs-22517R-HRP) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Pig, Horse.
How should bs-22517R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Zic2 Polyclonal Antibody, HRP Conjugated (bs-22517R-HRP)?
KLH conjugated synthetic peptide derived from human Zic2