CD4 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-22732R-APC-Cy7-100uL |
| Product Name | CD4 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Guinea Pig |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 920 |
| Swiss Prot | P01730 |
| Subcellular location | Cell membrane |
| Synonyms | CD4 (L3T4); CD4 antigen (p55); CD4 Antigen ; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4 ; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3 ; T cell surface antigen T4/Leu3; T Cell Surface Glycoprotein CD4; W3/25; W3/25 antigen; T-cell surface glycoprotein CD4 isoform 1 precursor; CD4_HUMAN. |
| Background | Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Subunit : Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CD4 Polyclonal Antibody, APC-Cy7 Conjugated (bs-22732R-APC-Cy7) validated for, and at what dilution?
bs-22732R-APC-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD4 Polyclonal Antibody, APC-Cy7 Conjugated (bs-22732R-APC-Cy7) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Guinea Pig.
How should bs-22732R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD4 Polyclonal Antibody, APC-Cy7 Conjugated (bs-22732R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human CD4
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-22732R-APC-Cy7)?
APC-Cy7 on bs-22732R-APC-Cy7: excitation / emission 650nm/780nm.