Topoisomerase I Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-23144R-Biotin-100uL |
| Product Name | Topoisomerase I Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Topoisomerase I |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 7150 |
| Swiss Prot | P11387 |
| Synonyms | DNA topoisomerase 1; DNA topoisomerase I; NUP98 fusion gene; TOP 1; TOP I; TOP1; TOP1_HUMAN; TOPI; Topoisomerase 1; Topoisomerase1; TopoisomeraseI; Type I DNA topoisomerase. |
| Background | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | =1:500-2000 |
| IHC-P | IHC-P=1:100-500 |
| IHC-F | IHC-F=1:100-500 |
Frequently asked questions
Which applications is Topoisomerase I Polyclonal Antibody, Biotin Conjugated (bs-23144R-Biotin) validated for, and at what dilution?
bs-23144R-Biotin is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Topoisomerase I Polyclonal Antibody, Biotin Conjugated (bs-23144R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-23144R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Topoisomerase I Polyclonal Antibody, Biotin Conjugated (bs-23144R-Biotin)?
KLH conjugated synthetic peptide derived from human Topoisomerase I