Topoisomerase I Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-23144R-Cy5.5-100uL |
| Product Name | Topoisomerase I Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Topoisomerase I |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7150 |
| Swiss Prot | P11387 |
| Synonyms | DNA topoisomerase 1; DNA topoisomerase I; NUP98 fusion gene; TOP 1; TOP I; TOP1; TOP1_HUMAN; TOPI; Topoisomerase 1; Topoisomerase1; TopoisomeraseI; Type I DNA topoisomerase. |
| Background | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | =1:500-2000 |
| IF | IF=1:100-500 |
Frequently asked questions
Which applications is Topoisomerase I Polyclonal Antibody, Cy5.5 Conjugated (BS-23144R-CY5-5) validated for, and at what dilution?
BS-23144R-CY5-5 is validated for WB (WB=1:500-2000); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Topoisomerase I Polyclonal Antibody, Cy5.5 Conjugated (BS-23144R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-23144R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Topoisomerase I Polyclonal Antibody, Cy5.5 Conjugated (BS-23144R-CY5-5)?
KLH conjugated synthetic peptide derived from human Topoisomerase I
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-23144R-CY5-5)?
Cy5.5 on BS-23144R-CY5-5: excitation / emission 675nm/694nm.