NFKB p65(acetyl K310) Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-23216R-PerCP-Cy5.5-100uL |
| Product Name | NFKB p65(acetyl K310) Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised acetylpeptide derived from human NFKBp65 around the phosphorylation site of Lys310 |
| Modification Site | k310 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5970 |
| Swiss Prot | Q04206 |
| Synonyms | NF-kB p65 (acetyl K310); NF-_B p65(acetyl K310); RELA(acetyl K310); NF kB P65; NF-kB p65; NFKBp65; NF-_Bp65; p65 NF kappaB; p65 NFkB; NFKBp65; RELA; Transcription Factor p65; v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)); V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; v rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A; p65NFKB; Avian reticuloendotheliosis viral (v rel) oncogene homolog A; MGC131774; NFKB 3; NFKB3; Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells; TF65_HUMAN. NF_B-p65; NF_B p65; NF _B-p65; NF_Bp65; |
| Background | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is NFKB p65(acetyl K310) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-23216R-PERCP-CY5-5) validated for, and at what dilution?
BS-23216R-PERCP-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NFKB p65(acetyl K310) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-23216R-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, GuineaPig.
How should BS-23216R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NFKB p65(acetyl K310) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-23216R-PERCP-CY5-5)?
KLH conjugated Synthesised acetylpeptide derived from human NFKBp65 around the phosphorylation site of Lys310
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-23216R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-23216R-PERCP-CY5-5: excitation / emission 482nm/694nm.