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Wnt3a Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Dog
  • Cow
  • Sheep
  • Pig
  • Rabbit
Overview
Catalog # bs-23278R-Cy5-100uL
Product Name Wnt3a Polyclonal Antibody, Cy5 Conjugated
Applications
WB
Reactivity Rat
Predicted Reactivity Human, Mouse, Dog, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Wnt3a
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 89780
Swiss Prot P56704
Subcellular location Extracellular, Secreted
Synonyms MGC119418; MGC119419; MGC119420; Protein Wnt 3a Precursor; Protein Wnt-3a; Wingless type MMTV integration site family member 3A; Wnt 3a; WNT3A protein.
Background Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Wnt3a Polyclonal Antibody, Cy5 Conjugated (bs-23278R-Cy5) validated for, and at what dilution?

bs-23278R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Wnt3a Polyclonal Antibody, Cy5 Conjugated (bs-23278R-Cy5) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Rabbit.

How should bs-23278R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Wnt3a Polyclonal Antibody, Cy5 Conjugated (bs-23278R-Cy5)?

KLH conjugated synthetic peptide derived from human Wnt3a

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-23278R-Cy5)?

Cy5 on bs-23278R-Cy5: excitation / emission 625,650nm/670nm.

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