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14-3-3 epsilon Polyclonal Antibody, AbBy Fluor-680 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Sheep
  • Chicken
  • Rabbit
Overview
Catalog # bs-2340R-BF680-100uL
Product Name 14-3-3 epsilon Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Sheep, Chicken, Rabbit
Specifications
Conjugation AbBy Fluor-680
Host Rabbit
Source KLH conjugated synthetic peptide derived from human 14-3-3E
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7531
Subcellular location Cytoplasm
Synonyms 14-3-3 epsilon; 14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 E; 14-3-3 protein epsilon; 14-3-3E; 1433E_HUMAN; KCIP 1; KCIP-1; KCIP1; MDCR; MDS; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; Protein kinase C inhibitor protein1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein epsilon polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide; YWHAE.
Background Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is 14-3-3 epsilon Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2340R-BF680) validated for, and at what dilution?

bs-2340R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does 14-3-3 epsilon Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2340R-BF680) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Chicken, Rabbit.

How should bs-2340R-BF680 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise 14-3-3 epsilon Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2340R-BF680)?

KLH conjugated synthetic peptide derived from human 14-3-3E

What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-2340R-BF680)?

AbBy Fluor-680 on bs-2340R-BF680: excitation / emission 679nm/702nm.

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