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alpha Internexin Polyclonal Antibody, Cy3 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-2408R-Cy3-100uL
Product Name alpha Internexin Polyclonal Antibody, Cy3 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human alpha Internexin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9118
Swiss Prot Q16352
Subcellular location Cytoplasm
Synonyms NEF5; NF-66; TXBP-1; Alpha-internexin; Alpha-Inx; 66 kDa neurofilament protein; Neurofilament-66; Neurofilament 5; INA
Background Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is alpha Internexin Polyclonal Antibody, Cy3 Conjugated (bs-2408R-Cy3) validated for, and at what dilution?

bs-2408R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does alpha Internexin Polyclonal Antibody, Cy3 Conjugated (bs-2408R-Cy3) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse.

How should bs-2408R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise alpha Internexin Polyclonal Antibody, Cy3 Conjugated (bs-2408R-Cy3)?

KLH conjugated synthetic peptide derived from human alpha Internexin

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-2408R-Cy3)?

Cy3 on bs-2408R-Cy3: excitation / emission 512,550nm/570,615nm.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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