Histone H3 (acetyl K4) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-24644R-RBITC-100uL |
| Product Name | Histone H3 (acetyl K4) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Histone H3 (acetyl K4) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8290 |
| Swiss Prot | Q16695 |
| Subcellular location | Nucleus |
| Synonyms | Histone H3(acetyl Lys4); Acetyl-Histone H3(Lys4); Acetyl-Histone H3(K4); H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; Histone H3; H3S; Histone H3-I/H3-II; Major histone H3; H3F; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H31_HUMAN. |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Histone H3 (acetyl K4) Polyclonal Antibody, RBITC Conjugated (bs-24644R-RBITC) validated for, and at what dilution?
bs-24644R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (acetyl K4) Polyclonal Antibody, RBITC Conjugated (bs-24644R-RBITC) react with?
Tested reactivity: Human.
How should bs-24644R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (acetyl K4) Polyclonal Antibody, RBITC Conjugated (bs-24644R-RBITC)?
KLH conjugated synthetic peptide derived from human Histone H3 (acetyl K4)
What are the excitation and emission wavelengths of the RBITC conjugate (bs-24644R-RBITC)?
RBITC on bs-24644R-RBITC: excitation / emission 540nm/625nm.