ACCN2 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2586R-Cy5-100uL |
| Product Name | ACCN2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ASIC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 41 |
| Subcellular location | Extracellular |
| Synonyms | Acid-sensing ion channel 1; ASICs1; Acid sensing ion channel 1; Acid sensing ion channel 1a protein; Acid sensing ion channel; AI843610; Amiloride sensitive cation channel 2 neuronal; ASIC; ASIC1; ASIC1A; B530003N02Rik; BNAC2; Brain sodium channel 2; Cation channel amiloride sensitive neuronal 2; hBNaC2. |
| Background | Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is ACCN2 Polyclonal Antibody, Cy5 Conjugated (bs-2586R-Cy5) validated for, and at what dilution?
bs-2586R-Cy5 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ACCN2 Polyclonal Antibody, Cy5 Conjugated (bs-2586R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Horse, Chicken.
How should bs-2586R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ACCN2 Polyclonal Antibody, Cy5 Conjugated (bs-2586R-Cy5)?
KLH conjugated synthetic peptide derived from human ASIC1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-2586R-Cy5)?
Cy5 on bs-2586R-Cy5: excitation / emission 625,650nm/670nm.