TSHB Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2676M-FITC-100uL |
| Product Name | TSHB Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Others |
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human TSHB |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7252 |
| Subcellular location | Cytoplasm |
| Synonyms | CHNG4; Thyroid stimulating hormone beta subunit; Thyroid stimulating hormone, beta precursor; Thyrotropin beta subunit; TSHB. |
| Background | Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TSHB Polyclonal Antibody, FITC Conjugated (bs-2676M-FITC) validated for, and at what dilution?
bs-2676M-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-2676M-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSHB Polyclonal Antibody, FITC Conjugated (bs-2676M-FITC)?
KLH conjugated synthetic peptide derived from human TSHB
What are the excitation and emission wavelengths of the FITC conjugate (bs-2676M-FITC)?
FITC on bs-2676M-FITC: excitation / emission 494nm/518nm.