phospho-IRS1 (Ser307) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2736R-FITC-100uL |
| Product Name | phospho-IRS1 (Ser307) Polyclonal Antibody, FITC Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to Ser302 in Mouse and Rat. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser307 [TE(p-S)IT] |
| Modification Site | Ser307 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3667 |
| Swiss Prot | P35568 |
| Subcellular location | Cell membrane |
| Synonyms | HIRS-1; Insulin receptor substrate 1; IRS-1; IRS1 |
| Background | May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity). |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-IRS1 (Ser307) Polyclonal Antibody, FITC Conjugated (bs-2736R-FITC) validated for, and at what dilution?
bs-2736R-FITC is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IRS1 (Ser307) Polyclonal Antibody, FITC Conjugated (bs-2736R-FITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-2736R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IRS1 (Ser307) Polyclonal Antibody, FITC Conjugated (bs-2736R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser307 [TE(p-S)IT]
What are the excitation and emission wavelengths of the FITC conjugate (bs-2736R-FITC)?
FITC on bs-2736R-FITC: excitation / emission 494nm/518nm.