phospho-ASK1 (Thr845) Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3031R-HRP-100uL |
| Product Name | phospho-ASK1 (Thr845) Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse ASK1 around the phosphorylation site of Thr845 |
| Modification Site | Thr845 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26408 |
| Swiss Prot | O35099 |
| Subcellular location | Cytoplasm |
| Synonyms | ASK; ASK1; Mekk5; MAPKKK5; 742452D2Rik; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Map3k5 |
| Background | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1). |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is phospho-ASK1 (Thr845) Polyclonal Antibody, HRP Conjugated (bs-3031R-HRP) validated for, and at what dilution?
bs-3031R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-ASK1 (Thr845) Polyclonal Antibody, HRP Conjugated (bs-3031R-HRP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-3031R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ASK1 (Thr845) Polyclonal Antibody, HRP Conjugated (bs-3031R-HRP)?
KLH conjugated synthetic phosphopeptide derived from mouse ASK1 around the phosphorylation site of Thr845