S-tag Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-33017R-Cy5-100uL |
| Product Name | S-tag Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide of S-tag |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Stag; S tag; Ribonuclease A; KETAAAKFERQHMDS. |
| Background | S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag. The amino acid sequence of the S-tag is: Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser. It is believed that the peptide with its abundance of charged and polar residues could improve solubility of proteins it is attached to[citation needed]. Moreover, the peptide alone is thought not to fold into a distinct structure. On DNA-level the S-tag can be attached to the N- or C-terminus of any protein. After gene expression, such a tagged protein can be detected by commercially available antibodies. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is S-tag Antibody, Cy5 Conjugated (bs-33017R-Cy5) validated for, and at what dilution?
bs-33017R-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does S-tag Antibody, Cy5 Conjugated (bs-33017R-Cy5) react with?
Tested reactivity: Species independent.
How should bs-33017R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise S-tag Antibody, Cy5 Conjugated (bs-33017R-Cy5)?
KLH conjugated synthetic peptide of S-tag
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-33017R-Cy5)?
Cy5 on bs-33017R-Cy5: excitation / emission 625,650nm/670nm.