Phospho-PERK (Thr980) Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3330r-pe-cy5-100uL |
| Product Name | Phospho-PERK (Thr980) Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980 |
| Modification Site | Thr980 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 13666 |
| Swiss Prot | Q9Z2B5 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Pek; Perk; AI427929; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; Eif2ak3 |
| Background | Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-PERK (Thr980) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3330R-PE-Cy5) validated for, and at what dilution?
bs-3330R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-PERK (Thr980) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3330R-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Rabbit.
How should bs-3330R-PE-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-PERK (Thr980) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3330R-PE-Cy5)?
KLH conjugated synthetic phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3330R-PE-Cy5)?
PE-Cy5 on bs-3330R-PE-Cy5: excitation / emission 488nm/670nm.