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Bcl-2 Polyclonal Antibody, FITC Conjugated

Applications

  • WB

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bs-33411R-FITC-100uL
Product Name Bcl-2 Polyclonal Antibody, FITC Conjugated
Applications
WB
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Bcl-2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 596
Swiss Prot P49950
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.
Background Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Bcl-2 Polyclonal Antibody, FITC Conjugated (bs-33411R-FITC) validated for, and at what dilution?

bs-33411R-FITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Bcl-2 Polyclonal Antibody, FITC Conjugated (bs-33411R-FITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bs-33411R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Bcl-2 Polyclonal Antibody, FITC Conjugated (bs-33411R-FITC)?

KLH conjugated synthetic peptide derived from human Bcl-2

What are the excitation and emission wavelengths of the FITC conjugate (bs-33411R-FITC)?

FITC on bs-33411R-FITC: excitation / emission 494nm/518nm.

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