Phospho-cdc25C (Thr48) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3482R-FITC-100uL |
| Product Name | Phospho-cdc25C (Thr48) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Thr48 |
| Modification Site | Thr48 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 995 |
| Swiss Prot | P30307 |
| Subcellular location | Nucleus |
| Synonyms | CDC25; PPP1R60; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; CDC25C |
| Background | Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-cdc25C (Thr48) Polyclonal Antibody, FITC Conjugated (bs-3482R-FITC) validated for, and at what dilution?
bs-3482R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-cdc25C (Thr48) Polyclonal Antibody, FITC Conjugated (bs-3482R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-3482R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-cdc25C (Thr48) Polyclonal Antibody, FITC Conjugated (bs-3482R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Thr48
What are the excitation and emission wavelengths of the FITC conjugate (bs-3482R-FITC)?
FITC on bs-3482R-FITC: excitation / emission 494nm/518nm.