CYP11A1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3608R-RBITC-100uL |
| Product Name | CYP11A1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1583 |
| Swiss Prot | P05108 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CYP11A; CYPXIA1; P45SCC; Cholesterol side-chain cleavage enzyme, mitochondrial; Cholesterol desmolase; Cytochrome P45 11A1; Cytochrome P45(scc); CYP11A1 |
| Background | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CYP11A1 Polyclonal Antibody, RBITC Conjugated (bs-3608R-RBITC) validated for, and at what dilution?
bs-3608R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYP11A1 Polyclonal Antibody, RBITC Conjugated (bs-3608R-RBITC) react with?
Tested reactivity: Rat.
How should bs-3608R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CYP11A1 Polyclonal Antibody, RBITC Conjugated (bs-3608R-RBITC)?
KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3608R-RBITC)?
RBITC on bs-3608R-RBITC: excitation / emission 540nm/625nm.