TSPO/PBR Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3674R-Cy3-100uL |
| Product Name | TSPO/PBR Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Peripheral type Benzodiazepine Receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1622 |
| Subcellular location | Cytoplasm |
| Synonyms | BPBS; BZRP; DBI; IBP; Isoquinoline carboxamide-binding protein; MBR; mDRC; Mitochondrial benzodiazepine receptor; PBR; PBS; Peripheral benzodiazepine receptor; Peripheral benzodiazepine receptor-related protein; Peripheral type benzodiazepine receptor; pk18; PKBS; PTBR; Ptbzr; PTBZR02; RATPTBZR02; translocator protein 18kDa; Translocator protein; TSPO; Tspo1; Benzodiazapine receptor peripheral; Benzodiazepine peripheral binding site. |
| Background | PBR (Peripheral type Benzodiazepine Receptor) is a ubiquitous protein (18 kDa) found in most steroidogenic tissues. It is located primarily in the outer mitochondrial membrane of steroid producing tissues and is found in association with a 34 kDa voltage dependent anion channel protein (VDAC). PBR interacts with some benzodiazepines and has different affinities than its endogenous counterpart. PBR appears to be a key factor in the transport of cholesterol from the outer to the inner mitochondrial membrane to permit the initiation of steroid hormone synthesis. It is thought to be part of the mitochondrial permeability transition pore (PT) and to be involved in apoptosis. It is speculated that patients with congenital lipoid adrenal hyperplasia, who cannot make any steroids, might have a genetic lesion in BZRP. A short form, PBRS is also expressed in the same tissues, but at a level about ten times that of PBR. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TSPO/PBR Polyclonal Antibody, Cy3 Conjugated (bs-3674R-Cy3) validated for, and at what dilution?
bs-3674R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSPO/PBR Polyclonal Antibody, Cy3 Conjugated (bs-3674R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig.
How should bs-3674R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSPO/PBR Polyclonal Antibody, Cy3 Conjugated (bs-3674R-Cy3)?
KLH conjugated synthetic peptide derived from human Peripheral type Benzodiazepine Receptor
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3674R-Cy3)?
Cy3 on bs-3674R-Cy3: excitation / emission 512,550nm/570,615nm.