Histone H3 (acetyl K9) Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3776R-Cy5-100uL |
| Product Name | Histone H3 (acetyl K9) Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This acetylation site is homologous across the species listed. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Pig, Rabbit, Fruit Fly |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic acetylpeptide derived from human Histone H3 around acetylation site of Lys9 |
| Modification Site | acetyl K9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3020 |
| Swiss Prot | P84243 |
| Subcellular location | Nucleus |
| Synonyms | H3F3; H3.3A; Histone H3.3; H3F3A; PP781 |
| Background | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Histone H3 (acetyl K9) Antibody, Cy5 Conjugated (bs-3776R-Cy5) validated for, and at what dilution?
bs-3776R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3 (acetyl K9) Antibody, Cy5 Conjugated (bs-3776R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Rabbit, Fruit Fly.
How should bs-3776R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3 (acetyl K9) Antibody, Cy5 Conjugated (bs-3776R-Cy5)?
KLH conjugated synthetic acetylpeptide derived from human Histone H3 around acetylation site of Lys9
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3776R-Cy5)?
Cy5 on bs-3776R-Cy5: excitation / emission 625,650nm/670nm.