PGP9.5 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3806R-BF750-100uL |
| Product Name | PGP9.5 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Cow |
| Predicted Reactivity | Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PGP9.5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7345 |
| Swiss Prot | P09936 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; UCHL1 |
| Background | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PGP9.5 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3806R-BF750) validated for, and at what dilution?
bs-3806R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PGP9.5 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3806R-BF750) react with?
Tested reactivity: Human, Mouse, Rat, Cow. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse, GuineaPig.
How should bs-3806R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PGP9.5 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3806R-BF750)?
KLH conjugated synthetic peptide derived from human PGP9.5
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-3806R-BF750)?
AbBy Fluor-750 on bs-3806R-BF750: excitation / emission 749nm/775nm.