NCF1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3886R-PerCP-Cy5.5-100uL |
| Product Name | NCF1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NCF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 653361 |
| Swiss Prot | P14598 |
| Subcellular location | Cytoplasm |
| Synonyms | NCF1A; NOXO2; p47phox; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1 |
| Background | NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NCF1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-3886R-PERCP-CY5-5) validated for, and at what dilution?
BS-3886R-PERCP-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NCF1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-3886R-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog.
How should BS-3886R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NCF1 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-3886R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human NCF1
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-3886R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-3886R-PERCP-CY5-5: excitation / emission 482nm/694nm.