phospho-GSK3 Beta (Tyr216) Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4079R-BF555-100uL |
| Product Name | phospho-GSK3 Beta (Tyr216) Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human GSK3 Beta around the phosphorylation site of Tyr216 |
| Modification Site | Tyr216 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2932 |
| Synonyms | GSK3BTyr216; GSK3BY216; p-GSK-3 BetaTyr216; p-GSK-3 betaY216; Glycogen synthase kinase 3 beta; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B_HUMAN. |
| Background | Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is phospho-GSK3 Beta (Tyr216) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-4079R-BF555) validated for, and at what dilution?
bs-4079R-BF555 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-GSK3 Beta (Tyr216) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-4079R-BF555) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should bs-4079R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-GSK3 Beta (Tyr216) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-4079R-BF555)?
KLH conjugated synthetic phosphopeptide derived from human GSK3 Beta around the phosphorylation site of Tyr216
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-4079R-BF555)?
AbBy Fluor-555 on bs-4079R-BF555: excitation / emission 553nm/568nm.