ZEB1/NIL2A Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4187R-Cy3-100uL |
| Product Name | ZEB1/NIL2A Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Specificity | Antibody will bind to Zinc finger E-box-binding homeobox 1 & 2 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZEB1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6935, 9839 |
| Swiss Prot | P37275, O60315 |
| Subcellular location | Nucleus |
| Synonyms | AREB 6; AREB6; BZP; Delta crystallin enhancer binding factor 1; DELTA EF1; FECD6; MGC133261; Negative regulator of IL 2; Negative regulator of IL2; NIL 2 A; NIL 2 A zinc finger protein; NIL 2A; NIL-2-A zinc finger protein; NIL2A; Posterior polymorphous corneal dystrophy 3; PPCD3; Represses interleukin 2 expression; TCF 8; TCF-8; TCF8; Transcription factor 8 represses interleukin 2 expression; Transcription factor 8; ZEB 1; ZEB; ZEB1; ZEB1_HUMAN; ZFHEP; ZFHX 1A; ZFHX1A; Zinc finger E box binding homeobox 1; Zinc finger E-box-binding homeobox 1; Zinc finger homeodomain enhancer binding protein; Smad Interacting Protein 1 SIP 1; SIP1 SIP-1; Smad-interacting protein 1; SMADIP 1; SMADIP1; ZEB 2; ZEB2; ZEB2_HUMAN; Zfhx1b; ZFHX1B protein; Zfx1b; Zinc finger E box binding protein 2; Zinc finger E-box-binding homeobox 2; Zinc finger homeobox 1b; zinc finger homeobox protein 1; Zinc finger homeobox protein 1b. |
| Background | ZEB1 Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. ZEB2 is a Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | FCM1:20-100 |
Frequently asked questions
Which applications is ZEB1/NIL2A Polyclonal Antibody, Cy3 Conjugated (bs-4187R-Cy3) validated for, and at what dilution?
bs-4187R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZEB1/NIL2A Polyclonal Antibody, Cy3 Conjugated (bs-4187R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Chicken, Rabbit.
How should bs-4187R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZEB1/NIL2A Polyclonal Antibody, Cy3 Conjugated (bs-4187R-Cy3)?
KLH conjugated synthetic peptide derived from human ZEB1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4187R-Cy3)?
Cy3 on bs-4187R-Cy3: excitation / emission 512,550nm/570,615nm.