ADAMTS4 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4191R-HRP-100uL |
| Product Name | ADAMTS4 Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Specificity | Due to the similarity this protein to ADAMTS1, there is a chance that this antibody may have a cross-reactivity with this protein, based upon a 73% sequence similarity in the epitope region. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ADAMTS4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9507 |
| Swiss Prot | O75173 |
| Subcellular location | Secreted |
| Synonyms | ADMP-1; ADAMTS-2; ADAMTS-4; A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS 4; ADAM-TS4; Aggrecanase-1; ADAMTS4; KIAA0688; UNQ769/PRO1563 |
| Background | Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is ADAMTS4 Polyclonal Antibody, HRP Conjugated (bs-4191R-HRP) validated for, and at what dilution?
bs-4191R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ADAMTS4 Polyclonal Antibody, HRP Conjugated (bs-4191R-HRP) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Rabbit.
How should bs-4191R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ADAMTS4 Polyclonal Antibody, HRP Conjugated (bs-4191R-HRP)?
KLH conjugated synthetic peptide derived from human ADAMTS4