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VAPB Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-4227R-FITC-100uL
Product Name VAPB Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human VAPB
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9217
Subcellular location Cytoplasm
Synonyms ALS 8; ALS8; D2Abb2e; UNQ484/PRO983; Vamp 33b; VAMP associated 33 kDa protein; VAMP associated protein B and C; VAMP associated protein B; VAMP associated protein B/C; VAMP associated protein C; VAMP B; VAMP B VAMP C; VAMP B/VAMP C; VAMP C; VAMP vesicle associated membrane protein associated protein B and C; Vamp33b; VAMPB; VAMPB/VAMPC; VAMPC; VAP 33b; VAP B; VAP B/VAP C; VAP C; VAP33b; VAPB/VAPC; VAPC antibody Vesicle associated membrane protein associated protein B and C; Vesicle associated membrane protein associated protein B/C; VAPB_HUMAN.
Background VAPB contains 1 MSP domain and it may play a role in vesicle trafficking. Defects in VAPB are a cause of proximal adult autosomal dominant spinal muscular atrophy [MIM:182980]; also called late onset spinal muscular atrophy Finkel type. Spinal muscular atrophies are neurodegenerative disorders characterized by degeneration of lower motor neurons, leading to progressive paralysis muscular atrophy. This form is a late adult onset form of the disease (after age 20 years). The patients show a benign course, most of them remaining ambulatory 10 to 40 years after clinical onset.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is VAPB Polyclonal Antibody, FITC Conjugated (bs-4227R-FITC) validated for, and at what dilution?

bs-4227R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does VAPB Polyclonal Antibody, FITC Conjugated (bs-4227R-FITC) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-4227R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise VAPB Polyclonal Antibody, FITC Conjugated (bs-4227R-FITC)?

KLH conjugated synthetic peptide derived from human VAPB

What are the excitation and emission wavelengths of the FITC conjugate (bs-4227R-FITC)?

FITC on bs-4227R-FITC: excitation / emission 494nm/518nm.

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