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Beta tubulin Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-4511R-Cy7-100uL
Product Name Beta tubulin Polyclonal Antibody, Cy7 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human tubulin Beta
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 203068
Swiss Prot P07437
Subcellular location Cytoplasm
Synonyms M4; TUBB1; TUBB5; CDCBM6; OK/SW-cl.56; Tubulin beta chain; Tubulin beta-5 chain; TUBB
Background Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Beta tubulin Polyclonal Antibody, Cy7 Conjugated (bs-4511R-Cy7) validated for, and at what dilution?

bs-4511R-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Beta tubulin Polyclonal Antibody, Cy7 Conjugated (bs-4511R-Cy7) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-4511R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Beta tubulin Polyclonal Antibody, Cy7 Conjugated (bs-4511R-Cy7)?

KLH conjugated synthetic peptide derived from human tubulin Beta

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-4511R-Cy7)?

Cy7 on bs-4511R-Cy7: excitation / emission 743nm/767nm.

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