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CSFV E2 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB

Reactivity

  • CSFV
Overview
Catalog # bs-4527R-Cy5.5-100uL
Product Name CSFV E2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB
Specificity The immunogen for this antibody is sourced strain Brescia.
Reactivity CSFV
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from CSFV Envelope glycoprotein E2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Swiss Prot P21530
Subcellular location Cell membrane
Synonyms polyprotein [Classical swine fever virus]; polyprotein; CSFV polyprotein; E2; Envelope glycoprotein E2; structural protein E2 [Classical swine fever virus]; POLG_CSFVA.
Background Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it?s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins.Envelope glycoprotein E2 exists in the form of homodimer; this protein is cleaved from the polyprotein by a cellular signal peptidase.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is CSFV E2 Polyclonal Antibody, Cy5.5 Conjugated (BS-4527R-CY5-5) validated for, and at what dilution?

BS-4527R-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CSFV E2 Polyclonal Antibody, Cy5.5 Conjugated (BS-4527R-CY5-5) react with?

Tested reactivity: CSFV.

How should BS-4527R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CSFV E2 Polyclonal Antibody, Cy5.5 Conjugated (BS-4527R-CY5-5)?

KLH conjugated synthetic peptide derived from CSFV Envelope glycoprotein E2

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4527R-CY5-5)?

Cy5.5 on BS-4527R-CY5-5: excitation / emission 675nm/694nm.

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