Bcl-2 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4563R-Cy5-100uL |
| Product Name | Bcl-2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Bcl-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 596 |
| Swiss Prot | P10415 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Bcl-2; PPP1R5; Apoptosis regulator Bcl-2; BCL2 |
| Background | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Bcl-2 Polyclonal Antibody, Cy5 Conjugated (bs-4563R-Cy5) validated for, and at what dilution?
bs-4563R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bcl-2 Polyclonal Antibody, Cy5 Conjugated (bs-4563R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-4563R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bcl-2 Polyclonal Antibody, Cy5 Conjugated (bs-4563R-Cy5)?
KLH conjugated synthetic peptide derived from human Bcl-2
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-4563R-Cy5)?
Cy5 on bs-4563R-Cy5: excitation / emission 625,650nm/670nm.