CA153 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4763R-RBITC-100uL |
| Product Name | CA153 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MUC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4582 |
| Subcellular location | Cytoplasm, Nucleus, Secreted, Cell membrane |
| Synonyms | Breast carcinoma associated antigen DF3; Breast carcinoma associated antigen DF3; Breast carcinoma-associated antigen DF3; CA 15 3; CA 15-3; CA15 3; CA15 3 antigen; CA15.3; Cancer antigen 15-3; Carcinoma associated mucin; Carcinoma associated mucin; Carcinoma-associated mucin; CD 227; CD227; CD227; CD227 antigen; DF3 antigen; DF3 antigen; EMA; EMA; Episialin; Episialin; Epithelial membrane antigen; Epithelial membrane antigen; Epithelial mucin tandem repeat sequence; H23 antigen; H23 antigen; H23AG; H23AG; HGNC:7508; KL 6; KL-6; KL6; Krebs von den Lungen-6; MAM 6; MAM6; MUC 1; MUC 1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1; MUC1-alpha; MUC1-beta; MUC1-CT; MUC1-NT; MUC1/ZD; MUC1_HUMAN; Mucin 1; Mucin 1 precursor; Mucin 1 transmembrane; Mucin-1 subunit beta; Mucin1; Mucin1; Peanut reactive urinary mucin; Peanut reactive urinary mucin; Peanut-reactive urinary mucin; PEM; PEMT; PEMT; Polymorphic epithelial mucin; Polymorphic epithelial mucin; PUM; PUM; Tumor associated epithelial membrane antigen; Tumor associated epithelial membrane antigen; Tumor associated epithelial mucin; Tumor associated mucin; Tumor associated mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin. |
| Background | MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is CA153 Polyclonal Antibody, RBITC Conjugated (bs-4763R-RBITC) validated for, and at what dilution?
bs-4763R-RBITC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CA153 Polyclonal Antibody, RBITC Conjugated (bs-4763R-RBITC) react with?
Tested reactivity: Human.
How should bs-4763R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CA153 Polyclonal Antibody, RBITC Conjugated (bs-4763R-RBITC)?
KLH conjugated synthetic peptide derived from human MUC1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-4763R-RBITC)?
RBITC on bs-4763R-RBITC: excitation / emission 540nm/625nm.