CD4 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4817R-Cy5.5-100uL |
| Product Name | CD4 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 920 |
| Swiss Prot | P01730 |
| Subcellular location | Cell membrane |
| Synonyms | CD4mut; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4 |
| Background | Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is CD4 Polyclonal Antibody, Cy5.5 Conjugated (BS-4817R-CY5-5) validated for, and at what dilution?
BS-4817R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD4 Polyclonal Antibody, Cy5.5 Conjugated (BS-4817R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-4817R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD4 Polyclonal Antibody, Cy5.5 Conjugated (BS-4817R-CY5-5)?
KLH conjugated synthetic peptide derived from human CD4
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4817R-CY5-5)?
Cy5.5 on BS-4817R-CY5-5: excitation / emission 675nm/694nm.