CD274 Polyclonal Antibody, PerCP-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4941r-percp-cy7-100uL |
| Product Name | CD274 Polyclonal Antibody, PerCP-Cy7 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PerCP-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD274 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29126 |
| Subcellular location | Cell membrane |
| Synonyms | B7-H1; PD-L1; CD274; Programmed cell death 1 ligand 1; Programmed death ligand 1; PD-L1; PDCD1 ligand 1; B7 homolog 1; B7-H1; Programmed death ligand 1; PD1L1_HUMAN. |
| Background | Programmed cell death 1 ligand 1(Programmed death ligand 1; PD-L1; PDCD1 ligand 1; B7 homolog 1; B7-H1; CD274) involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibit T-cell proliferation and cytokine production. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is CD274 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-4941R-PerCP-Cy7) validated for, and at what dilution?
bs-4941R-PerCP-Cy7 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD274 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-4941R-PerCP-Cy7) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Rabbit.
How should bs-4941R-PerCP-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD274 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-4941R-PerCP-Cy7)?
KLH conjugated synthetic peptide derived from human CD274
What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bs-4941R-PerCP-Cy7)?
PerCP-Cy7 on bs-4941R-PerCP-Cy7: excitation / emission 482nm/780nm.