H1N1 Nucleoprotein Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4976R-Biotin-100uL |
| Product Name | H1N1 Nucleoprotein Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Virus |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Influenza A virus (strain A/Russia:St.Petersburg/8/2006 H1N1) Nucleoprotein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Synonyms | Nucleoprotein; NP; Nucleocapsid protein; Protein N; Influenza A virus H1N1; H3N2 Nucleoprotein; H9N2 Nucleoprotein; H2N2 Nucleoprotein; H3N8 Nucleoprotein; H7N7 Nucleoprotein; H5N1 Nucleoprotein. |
| Background | Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus. |
| Application Dilution | |
| WB | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is H1N1 Nucleoprotein Polyclonal Antibody, Biotin Conjugated (bs-4976R-Biotin) validated for, and at what dilution?
bs-4976R-Biotin is validated for WB (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does H1N1 Nucleoprotein Polyclonal Antibody, Biotin Conjugated (bs-4976R-Biotin) react with?
Tested reactivity: Virus.
How should bs-4976R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise H1N1 Nucleoprotein Polyclonal Antibody, Biotin Conjugated (bs-4976R-Biotin)?
KLH conjugated synthetic peptide derived from Influenza A virus (strain A/Russia:St.Petersburg/8/2006 H1N1) Nucleoprotein