Phospho-MAPKAPK2 (Ser272) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-5503R-RBITC-100uL |
| Product Name | Phospho-MAPKAPK2 (Ser272) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat, Bovine |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of Ser272 |
| Modification Site | Ser272 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9261 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MAPKAPK2phospho S272;P-MAPKAPK2Ser272; MAP Kinase Activated Protein Kinase 2; MAPK activated protein kinase 2; MAPKAP kinase 2; MAPKAPK 2; MAPKAPK2; Mitogen Activated Protein Kinase Activated Protein Kinase 2; MAPK2_HUMAN; MK 2; MK2; MAPKAPK-2. |
| Background | MAP kinase activated protein kinase 2 (MAPKAP Kinase 2), also known as p45 hsp27 kinase, is a 45-54 kDa serine/threonine protein kinase that contains a proline rich sequence and two putative SH3 binding sites. MAPKAP Kinase 2 is activated in response to stress, IL1 and TNF, possibly catalyzed by p38/Hog dependent phosphorylation. One of the major substrates of MAPKAP Kinase 2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. MAPKAP2 is implicated in several disorders including ischemic brain injury and heart failure and has been shown to be important in regulating stress resistance and the production of TNF alpha. |
| Application Dilution | |
| WB | =1:500-2000 |
| IF | IF=1:100-500 |
Frequently asked questions
Which applications is Phospho-MAPKAPK2 (Ser272) Polyclonal Antibody, RBITC Conjugated (bs-5503R-RBITC) validated for, and at what dilution?
bs-5503R-RBITC is validated for WB (WB=1:500-2000); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MAPKAPK2 (Ser272) Polyclonal Antibody, RBITC Conjugated (bs-5503R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat, Bovine.
How should bs-5503R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MAPKAPK2 (Ser272) Polyclonal Antibody, RBITC Conjugated (bs-5503R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of Ser272
What are the excitation and emission wavelengths of the RBITC conjugate (bs-5503R-RBITC)?
RBITC on bs-5503R-RBITC: excitation / emission 570nm/595nm.