phospho-TSC1 (Ser505) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-5600R-BF750-100uL |
| Product Name | phospho-TSC1 (Ser505) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human TSC1 around the phosphorylation site of Ser505 [FD(p-S)PF] |
| Modification Site | Ser505 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7248 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | TSC1phospho S505; LAM; TSC1; Tuberous sclerosis 1 protein. |
| Background | Hamartin, or TSC1, is a suspected tumor suppressor implicated in the disease tuberous sclerosis 1. It is a negative regulator of cell division controlling the transition from G0/G1 to S phase, and it seems to act through the phosphatidylinositol 3 kinase/Akt pathway. TSC1 interacts with tuberin m(TSC2), which is thought to be a GAP (GTPase Activating Protein) for the Rap1 and Rab5 small G Proteins. The Hamartin/Tuberin complex has been shown to inhibit mTor. Hamartin has also been shown to interact with ERM (Ezrin-Radixin-Moesin) proteins and with F-actin, suggesting a role for TSC proteins in modulation of cell adhesion and morphology. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-TSC1 (Ser505) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-5600R-BF750) validated for, and at what dilution?
bs-5600R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-TSC1 (Ser505) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-5600R-BF750) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-5600R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-TSC1 (Ser505) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-5600R-BF750)?
KLH conjugated synthetic phosphopeptide derived from human TSC1 around the phosphorylation site of Ser505 [FD(p-S)PF]
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-5600R-BF750)?
AbBy Fluor-750 on bs-5600R-BF750: excitation / emission 749nm/775nm.